特集 X線の次
どうするタンパク質結晶構造解析
―生体高分子構造研究におけるX線結晶学―
2023 年 65 巻 1 号 p. 55-63
詳細
2023 年 65 巻 1 号 p. 55-63
In modern life science research, structural information on proteins is indispensable for understanding the mechanisms of their biological functions. Many protein structures have been determined by X-ray crystallography, which has been developed in conjunction with molecular biology experiments and synchrotron radiation development. Since the Nobel Prize in Chemistry in 2017, the rapid progress of cryo-EM hardware and analysis software has led to the use of cryo-EM single-particle analysis for structural analysis. In this article, we compare the features of protein crystallography and cryo-EM single-particle analysis and discuss the best method for protein crystallography.
