ヒト由来グルコキナーゼの結晶構造解析

抄録

Glucokinase is a cytoplasmic enzyme that phosphorylates glucose and triggers glucose utilization and metabolism. Glucokinase expressed in liver and pancreas is thought to be the glucose sensor controlling plasma glucose levels. The role of glucokinase as a glucose sensor is due to its allosteric properties. In order to elucidate the molecular mechanism of glucose sensor, we analyzed the crystal structures of human glucokinase in both its active and inactive forms. Crystal structures suggest that the allosteric regulation of monomeric glucokinase obeys the“mnemonical mechanism”rather than the well-known concerted model.