トリクロマティック多波長異常分散法およびデータ収集の自動化に関する開発研究

抄録

At the SPring-8, we have developed and improved protein crystallography beamlines to achieve the data collection for High-Throughput Protein Crystallography. RIKEN Structural Biology Beamline I (BL45XU) is the first brunched Undulator beamline. The PX-station of BL45XU introduced the Trichromatic concept that optimizes for the multi-wavelength anomalous diffraction (MAD) method with three-colored and coaxial synchrotron X-ray beams. The concept enables rapid and flexible switching of three defined wavelengths and can be collected MAD data taking into account syatematic errors such as radiation damage. RIKEN Structural Genomics Beamlines (BL26B1 and BL26B2) have implemented the automatic operation for rapid data collection of a vast amount of protein crystals to contribute to the structural genomics research. The automation has been achieved by developing the centralized control software BSS. The nonstop data collections for multiple samples have been achieved by developing the new-concept sample changer SPACE. The automatic operation has been satisfactorily performed, for more than two years since 2003.