Abstract
The objective of this study was to establish the technology to identify wheat and barley cultivars by random amplified polymorphic DNA(RAPD) analysis to improve the quality of wheat and barley in Tochigi prefecture and to prevent seed contamination in the foundation seed and the stock seed. A total of 17 wheat and 19 barley cultivars in the Kanto region were examined. Wheat cultivars could be identified individually by RAPD analysis using 5 random primers, followed by the electrophoresis of the DNA fragments on 1.5% agarose gels and staining with ethidium bromide. Barley cultivars could be identified individually by RAPD analysis using 6 random primers. A polymorphism was observed among stock seeds collected from various areas in the old wheat cultivar, Norin 61, that was bred in 1945. No polymorphism was observed among F13 pedigrees of two-rowed barley, Kanto Nijo 35, showing that they were fixed.
