Abstract
1) The embryo transplantation was carried out with immature grains of wheat and barley. After the operated seeds ripened, the transplanted embryos, were excised again, and examinations were made on the differentiation of several Organs : leaf, epiblast, ventral scale, radicle, coleorhyza, scutellum, axillary bud of coleoptile and length of epithelial cell. 2) In many transplanted embryos the differentiation of third foliage leaf was observed, which was equally ascertained in mature embryos of intact wheat seeds (Fig. 1). Differentiation and growth of the other organs proceeded similarly to those of embryos of intact seeds (Table 1), as in the case of leaves. High positive correlation was found between the number of leaf and the length of embryo (Fig. 2). 3) From these facts, it is presumable that the increase of embryo size is accompanied by the progress of differentiation of the organs of embryo, and that even after transplantation the embryonal growth proceeds further without developing into seedling. Therefore, it may be possible that transplanted embryos are provided with nutritional substances by the host endosperm. These results seem to have a great significance for the consideration of the influences of endosperm upon the transplanted embryo.
