Abstract
In the endosperm of two-rowed barley, thick, 3-4 cell layers' aleurone layers are formed in the peripheral, but they are not formed in the particular area facing to the nucellar projection (Fig. 1). Almost all cells of one to five layers in the area, which is lacking in aleurone, differentiated to the transfer cells on 6-8 days after anthesis. At first, slight and many wall ingrowth appeared and the cell walls were looked like the teeth of a saw (Figs. 3 and 4). About a week after, the individual tooth elongated and, as remarkably developed, it became sometimes to bridge-like form across the cell (Figs. 5 and 6). Well developed transfer cells abound in the front area just opposite to nucellar projection. However, it is noticeable that a few highly- developed transfer cells were found in group (showed with arrow in Fig. 7) in both sides of the front area. In the last stage of ripening, the transfer cells were filled up with reserve substances, therefore, the characteristic wall ingrowth became not distinguishable (Fig. 9). The area of transfer cells facing to nucellar projection seems to play an important role in the translocation of reserve substances into endosperm in two-rowed barley which the endosperm is encircled with mulch-layers and thick-walled aleurone cells.
