Abstract
A device and operation for preparing of parallel thin sections of undecalcified dental hard tissues have been described.
The principle of the sectioning operation is to cut the sample with a circular diamond blade while moving the sample through the blade. The specimens are cut as a rule without preliminary embedding and taken off at the time of every completion of the cuts to avoid secondary damage. The basic principle of the grinding consists of rotating a turntable carring a grinding stone and sections affixed on a specimen-holder to which stroke motion is given with a spindle together. After all operations the thin section is immersed into xylene and separated from the specimen-holder.
Using sectioning techniques, although 100μ-thick section may be occationally obtainable, whole undecalcified teeth can be routinely sectioned at a thickness of 200μ. After then, the grinding operation can provide thinner section at 20μ to 30μ thickness, which is parallel within 3μ. The sections thus prepared are suitable for immediate observation under microscope and are in original state, since no preliminary decalcification, embedding is used.
