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Volume 20 (1970 - 197･･･
- Issue 3 Pages 215-
- Issue 2 Pages 113-
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Volume 20, Issue 2
Displaying 1-7 of 7 articles from this issue
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Takehiko SUETAKA1970 Volume 20 Issue 2 Pages 113-162
Published: 1970
Released on J-STAGE: March 02, 2010
JOURNAL FREE ACCESSGenerally speaking, operational efficiency relates to the thermal conditions in a given environment, and there exist many thesises on the optimum thermal conditions necessary for various kinds of operations. Only one thesis, however, written by Junji Sunouchi, specifically deals with the problems of dentists in this field.
In this thesis, the author is interested in ascertaining the optimum thermal conditions in a dental operating room through investigations of actual thermal conditions in different dental operating rooms and measurement of the physiological relations between thermal conditions and the operational efficiency of the dentists.
This thesis is divided into four chapters: In the first chapter, the author investigates the utilization of space heating-cooling equipments in about 500 dental operating rooms throughout the country; in the second chapter, he investigates the thermal conditions in the dental operating rooms of 29 selected dentists residing in Tokyo and Niigata (which belong to different climatological patterns in Japan) and determines the poysiological effect of the thermal conditions on them; in the third chapter, he determines physiological effect on the thermal conditions on 8 dentists in an experimentally created environment; and in the fourth chapter, the author describes his conclusions as to the various relationships between room temperature and skin temperature, and thermal sensations, skin sensations and sensations of pleasantness. According to his findings, the range of the optimum temperature in the dental room is as follows:
Spring Summer Fall Winter
Tokyo 20-26°C 22-26°C 19-25°C 18-23°C
Niigata 18-24°C 22-25°C 19-24°C 19-25°C
In this thesis, the author finds the optimum thermal conditions necessary for the dental operating room. He also forms tentative donclusions as to the extent of the “uncomfort zone” in terms of temperature and humidity conditions. This thesis includes 30figures, 62 tables, and a list of 99 books and articles directly quoted.View full abstractDownload PDF (9650K) -
Masao ONISI, Miyoko OKANO1970 Volume 20 Issue 2 Pages 163-172
Published: 1970
Released on J-STAGE: July 27, 2010
JOURNAL FREE ACCESSTwo filamentous organisms were screened from bacterial plaque by the use of propionate medium containing propionic acid in a concentration of 0.5% in common nutrient agar adjusted to pH7.0. The first one, which grew in a spider form colony, stained gram positive and showed characteristic granular growth and produced a mucinous precipitate in broth. Soluble starch was quickly hydrolized to produce acid. In spite of colonial similarity to Bacterionema matruchotti, their biological characteristics would be close to Odontomyces viscosus. This was conveniently called“ Granular organism” by this department. The heat killed cells were capable of producing skin lesions, by either Schwartzman or Arthus procedure, in rabbits, guinea pigs, and rats. Another colony, having a hair-pencil appearance, stained gram negative and developed long straight filaments. These should be idenifiable to Leptotrichia species, but extreme difficulty in producing pure stock cultures prohibited obtaining enough descriptive information for their taxonomy.View full abstractDownload PDF (9951K) -
3rd Report: Fluoride analysis of surface enamel using acid etching in vitro and in situ (Biopsy) with particular references to topical fluoride applicationTakeshi KONDO1970 Volume 20 Issue 2 Pages 173-179
Published: 1970
Released on J-STAGE: November 26, 2010
JOURNAL FREE ACCESSIn order to compare the effectiveness of several topical fluoride application procedures, the enamel biopsy was applied to determine the removed fluoride and calcium by acid etching. The biopsy was carried out with the following technique; in the middle area of the labial surface of deciduous or permanent incisors, the acid etching was made with a filter paper disc (∅ 5mm) containing perhydrochloric acid (2M). The disc was removed and shaken in distilled water and the fluoride was determined by a fluoride electrode using a microsample dish.
The difference between the mean fluoride contents of deciduous and permanent teeth was not significant ranging from 363 to 540 ppm. There was no increase of fluoride levels of surface enamel with the increase of age.
The effect of topical fluoride application was compared on extracted teeth between the pretreatment by either neutral or acidified sodium fluoride or stannous fluoride. The largest retention of fluoride in surface enamel was found after the treatment with phosphate-fluoride solution at pH 3 and the removed calcium by acid was also lowest by this procedure.View full abstractDownload PDF (2724K) -
Masao ONISI, Mitsuko KOSUGE1970 Volume 20 Issue 2 Pages 180-183
Published: 1970
Released on J-STAGE: March 02, 2010
JOURNAL FREE ACCESSIn the previous paper, the fact that dental tissue is stained brown in the caramelized glucose solution of alkaline reaction was assumed to be the result of Ca-saccharate formation. Present paper followed this phenomenon by determination of Ca and P in the solution. Every 300mg enamel and dentine powder obtained from freshly extracted bovine teeth was suspended on every 50ml solution at pH 7.5, consisting of either tris-buffer (B), mol sucrose solution in B (S) or autoclaved S solution (CS). After 5 days incuvation at 37°C, the solution was separated from the dental powder for the determination of Ca, P and Ca/P ratio. The remained dentinal powder was resuspended 6 times repeatedly and received similar treatment. Generally Ca/P ratio increased according to every procedure, but CS and S extracted more Ca to result larger ratio. This fact was assumed to be ascribed to the Ca-compound formation of sucrose and additional component of caramel. Ca and P obtained in every suspension dereased according to every suspending, as it would be assumed to be repeated extraction of soluble Ca and P in the dental tissues. Effectiveness of Ca extraction of each solutions CS>B>S in this order, while for P effectiveness was B>CS>S. Exceptionally larger amount of Ca was obtained at first extraction by three solutions. Finally, it was discussed that present knowledge on physicochemical changes proceeding in dental tissue placed in the physiologically alkaline medium would not be complete.View full abstractDownload PDF (606K) -
Akira TSUNEMITSU, Yoshifumi IWAMOTO, Tatsuo WATANABE, Toshiro YAMAZAKI ...1970 Volume 20 Issue 2 Pages 184-190
Published: 1970
Released on J-STAGE: March 02, 2010
JOURNAL FREE ACCESSConcerning the purification and amino acid composition of human saliva, Petit and Jollès (1963) first purified lysozyme from human whole saliva and reported that its amino acid composition was somewhat different from that of hen egg-white lysozyme. More recently, Balekjian, Hoerman and Berzinskas (1969) has demonstrated that the parotid lysozyme has a composition which varies especially with respect to tyrosine, cystine and glutamic acid content. This communication was designed to confirm the amino acid composition and molecular weight of lysozyme from human parotid saliva.
About eight liters of human parotid saliva were collected from healthy adults with the device according to the method of Keene. Parotid lysozyme was purified to 525-fold by the procedures involving ammonium sulfate fractionation, DEAE-cellulose and CM-cellulose column chromatography, Sephadex G-75 gel filtration and adsorption on Amberlite CG-50 resin. The homogeneity of the purified sample was determined by both ultracentrifugation and disc electrophoresis. It had a specific activity 3.5 times greater than that of hen egg-white lysozyme and an amino acid composition which varied especially in regard to tyrosine, serine and glutamic acid content. The molecular weight was estimated to be around 14, 300.View full abstractDownload PDF (2883K) -
Toshibumi ISHII, Shigeru YOSHIDA, Hideo OSHIO, Tatuo SHIRAISHI1970 Volume 20 Issue 2 Pages 191-200
Published: 1970
Released on J-STAGE: March 02, 2010
JOURNAL FREE ACCESSA device and operation for preparing of parallel thin sections of undecalcified dental hard tissues have been described.
The principle of the sectioning operation is to cut the sample with a circular diamond blade while moving the sample through the blade. The specimens are cut as a rule without preliminary embedding and taken off at the time of every completion of the cuts to avoid secondary damage. The basic principle of the grinding consists of rotating a turntable carring a grinding stone and sections affixed on a specimen-holder to which stroke motion is given with a spindle together. After all operations the thin section is immersed into xylene and separated from the specimen-holder.
Using sectioning techniques, although 100μ-thick section may be occationally obtainable, whole undecalcified teeth can be routinely sectioned at a thickness of 200μ. After then, the grinding operation can provide thinner section at 20μ to 30μ thickness, which is parallel within 3μ. The sections thus prepared are suitable for immediate observation under microscope and are in original state, since no preliminary decalcification, embedding is used.View full abstractDownload PDF (12209K) -
Fumio MATSUDAIRA1970 Volume 20 Issue 2 Pages 201-213
Published: 1970
Released on J-STAGE: March 02, 2010
JOURNAL FREE ACCESSSamples from dentinal carious lesions were collected in operative procedures as possible as aseptically. Obtained specimens were inoculated on geratin medium and incubated anaerobically. In the case of specimens from the carious lesions in the mouth, a total of 40 organisms were isolated from 9 teeth, and divided into four groups. Group 1 and Group 2 were 14 strains identified to Nocardia dentocariosus, Group 3 consisted from 18 strains was designated as Actinomyces species, and Group 4, 8 strains, was designated as Micrococcus species. From the extracted tooth, 13 strains of isolations were found from 10 teeth, being identified to N. dentocariosus and only one strain of isolations was Actinomyces species. Geratinolytic activity of N. dentocariosus was examined by means of plate method, test-tube method, and viscosity technique. It was found that the activity was distinctively increased when the incubation was carried in a geratin medium in addition of glucose 1%. Decreasing at viscosity of geratin medium is not considered to be dependent on acid or temparature during incubation, but on hydrolysis. It was found that geratinolytic activity of N. dentocariosus, M-10 strain was the most strong among the present isolates, and its ability was found in the supernatant of broth culture.View full abstractDownload PDF (4324K)
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