Simultaneous Quantification Analysis of Organochlorine Pesticides by Capillary Gas Chromatography with ECD Detection

—Studies on the Residual Pesticide Assay in Food—

Abstract

Simultaneous Quantification Method of organochlorine pesticides by gas chromatography (GC) using good separative capillary columns and⁶³Ni-ECD was studied. Twenty-one organochlorine compounds including DDTs, HCHs, chlordanes and dieldrin analogues, all of which had been reported to pollute environmental samples, in addition to two kinds of phthalic acid esters often contaminating sample in laboratory were separated by 100% dimethyl polysiloxane (DB-1^TM) capillary column.
Concentrations of all the pesticides in food could be accurately determined by using internal standards (IS), δ-HCH and o, p'-DDD, for the reason that these compounds had been seldom detected in various food. Coefficient of variation (percent relative standard deviation) for the peak areas of these pesticides assayed in repeated injection to GC resulted into the range from 0.68% to 4.73% when the areas were corrected on the basis of the magnitude of ISs areas, and into the range from 4.44% to 6.27% when uncorrected by the IS.
The elution pattern of the twenty-three compounds mentioned above in 14% cyanopropylphenyl-86% dimethyl polysiloxane (DB-1701^TM), and in 50% trifluoropropyl-50% methyl polysiloxane (DB-210^TM) capillary columns were also described in the text.