Abstract
In order to develope sensitive and automatic determination method of the β-galactosidase activity in umu-test, a flow-injection fluorometric system has been applied. In this system, β-galactosidase induced by umu-test in the Salmonella typhimurium strain TA1535/pSK1002 was reacted with 4-methylumbellif eryl-β-D-galactopyranoside (4MUG) as a substrate. The activity was measured as the fluoresence intensity of 4-methylumbellif eron which was the reaction product. Detectability of this method was confirmed by measurement of the dose response relationship using typical 4 mutagens: 4-nitroquinoline-N-oxide (4NQ0), benzo (a) pyrene (BaP), 2-aminoanthracene (2AA) and 2- [2-furyl] -3- [5-vitro-2-furyl] acrylamide (AF2) .
