Journal of Electrophoresis
Online ISSN : 1349-9408
Print ISSN : 1349-9394
ISSN-L : 1349-9394
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Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry-based biotyping of silk. I. Method development
Hideyuki KajiwaraRitsuko MurakamiKenichi Nakajima
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2021 Volume 65 Issue 1 Pages 39-47


A method for the analysis of silkworm (Bombyx mori L.) cocoons and silk fibers was established using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. This method was evaluated on the most popular four-way cross hybrid race Kinshu × Showa and its parent races Kinshu and Showa. Most of the peaks observed in the peptide mass fingerprints of the Kinshu × Showa cocoon were from the parent races Kinshu and Showa. Simultaneous acid cleavage of silk fibers at room temperature is a characteristic phenomenon that has not been observed in spider silk and animal hair.

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