Journal of Electrophoresis
Online ISSN : 1349-9408
Print ISSN : 1349-9394
ISSN-L : 1349-9394
Short communication
Proteomic analysis of spheroids of rhabdomyosarcoma cells cultured with decellularized muscle extracts
Yooksil SinTakuya OnoRyuto TsuchiyaRei NoguchiYuki YoshimatsuHidetaka KosakoTadashi Kondo
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Supplementary material

2022 Volume 66 Issue 1 Pages 1-4


The tumor microenvironment determines tumor characteristics, and its recapitulation via in vitro tissue culture conditions is necessary for better understanding of molecular mechanisms of tumor behaviors and development of novel therapy. Rhabdomyosarcoma is a mesenchymal tumor that originates limb muscles. To assess the possible utility of the decellularized materials that contain native components of the microenvironment for the optimal humanized spheroid formation, we demonstrated co-culture of decellularized muscle extracts and rhabdomyosarcoma cells. The decellularized muscle extracts were prepared using a detergent, solubilized via pepsin digestion, and used for the spheroid culture of rhabdomyosarcoma cells in a low-attachment tissue culture plate. Here, we confirmed that these extracts contained proteins whose expression was unique to muscles. When rhabdomyosarcoma cells formed spheroids with decellularized muscle extracts, they exhibited heterogeneous morphology and a unique protein expression pattern. We conclude that in vitro cancer models established using decellularized tissue extracts from the originating organ have the potential to recapitulate features of in vivo tumor cells by providing the components of the tumor microenvironment. The precise molecular mechanisms for the interaction of cells and decellularized tissues are worth further investigation.

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