Detection of Gene Mutations Possibly Related to Methanol Tolerance in Gluconobacter frateurii Mutant Gf398

抄録

Strain Gf398 is an improved methanol-tolerant mutant of Gluconobacter frateurii NBRC 103465. Comparative genome analysis of Gf398 and NBRC 103465 revealed 18 mutated genes as potentially related to the methanol tolerance of Gf398. These mutated genes included enzyme-related, cell membrane-associated, cellular maintenance, and hypothetical protein-encoding genes, as well as one regulatory gene (ompR). The methanol tolerances of a recombinant NBRC 103465 strain expressing mutated ompR and one expressing the wild-type ompR were compared; the mutant showed slightly better cell growth in 2% (v/v) methanol-containing glycerol medium.