Journal of Ecotechnology Research
Online ISSN : 1884-0388
Print ISSN : 1881-9982
ISSN-L : 1881-9982
Original Article
ヒメダカの鰓を用いた小核試験の確立と廃水試料への適用
楠井 隆史吉田 有良
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ジャーナル フリー

2004 年 10 巻 3 号 p. 125-130

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The micronuclei test with gills of Japanese medaka (Oryzias latipes) was developed by modification of the traditional method. The effectiveness of this assay was confirmed with medaka exposed to Benzo[a] pyren, and the time course and concentration-dependent patterns of micronuclei induction were discussed. Finally, this new method was applied to effluent samples from 3 landfill sites and 1 chemical factory. All of raw leachate samples induced micronuclei significantly, which were reduced through treatments. The fact that the significant induction of micronucleated cells was observed when fishes were exposed to lower concentration of effluents than that of LC50 suggests the importance of genotoxicity monitoring with this test.
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© 2004 特定非営利活動法人 エコテクノロジー研究会
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