Detection of Antibody to Japanese Encephalitis Virus ( JEV) by Enzyme-Linked Immunosorbent Assay (ELISA)

抄録

To measure levels of antibody to Japanese encephalitis virus (JEV) in equine serum, an enzyme-linked immunosorbent assay (ELISA) was applied. Purified JEV particles of BMIII strain, derived from Beijing-1 strain, were physically adsorbed onto an immunoplate and the ability to react with anti-JEV antibody was tested. Whole virus particles reacted with the antibody, but the NP-40 lysed antigen did not. The ELISA titer correlated with the virus neutralization (VN) titer and the hemagglutination inhibition (HI) titer, although correlation coefficients were not very high. When the time course of the sera of immunized horses was measured by these three assays, the ELISA response was detected later than the VN and HI responses in 3 of 4 horses. This system can be useful for screening antibody to JEV.