Isolation of Satellite Cells from Equine Skeletal Muscle

Chie SOETA; Keitaro YAMANOUCHI; Telhisa HASEGAWA; Nobushige ISHIDA; Harutaka MUKOYAMA; Hideaki TOJO; Chikashi TACHI

doi:10.1294/jes.9.97

NOTE

Isolation of Satellite Cells from Equine Skeletal Muscle

Chie SOETA, Keitaro YAMANOUCHI, Telhisa HASEGAWA, Nobushige ISHIDA, Harutaka MUKOYAMA, Hideaki TOJO, Chikashi TACHI

著者情報

Chie SOETA
Laboratory of Applied Genetics, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113, Japan
Keitaro YAMANOUCHI
Laboratory of Applied Genetics, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113, Japan
Telhisa HASEGAWA
Laboratory of Molecular and Cellular Biology, Equine Research Institute, Japan Racing Association, Utsunomiya 320, Japan
Nobushige ISHIDA
Laboratory of Molecular and Cellular Biology, Equine Research Institute, Japan Racing Association, Utsunomiya 320, Japan
Harutaka MUKOYAMA
Laboratory of Molecular and Cellular Biology, Equine Research Institute, Japan Racing Association, Utsunomiya 320, Japan
Hideaki TOJO
Laboratory of Applied Genetics, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113, Japan
Chikashi TACHI
Laboratory of Applied Genetics, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113, Japan

キーワード: equine, satellite cells, desmin, culture

ジャーナルフリー

1998 年 9 巻 3 号 p. 97-100

DOI https://doi.org/10.1294/jes.9.97

詳細

抄録

Satellite cells from equine soleus muscles were cultured and the expression of desmin, one of the markers of skeletal muscle cells, was examined. The presence of desmin was detected in some populations of mononucleated cells on day 3 of culture, indicating that equine satellite cells were successfully isolated. On day 5, desmin was localized at the edges of the elongated myotubes but when the equine myotubes matured, no particular localization of desmin expressed diffusely within the cells was seen. Although the significance of such changes in the localization of desmin within the differentiating equine skeletal muscle cells is unknown, these results suggested that desmin may be involved in the growth and development of skeletal muscle in this species.

責任著者(Corresponding author)

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