Abstract
A bacteriological study was carried out to evaluate media and culture-conditions for isolation, growth and storage of Haemophilus equigenitalis. Chocolate agar plates made from eugon, modified Thayer-Martin and thiol agar media showed a better growth of bacteria than those made from any other medium and could be applied to primary isolation. The best growth of bacteria was observed on any agar media in an atmosphere containing 10% CO2 and 75% N2. Of 8 kinds of liquid or semisolid media, thiol broth and cystine trypticase showed an excellent growth of bacteria and a bacterial count ranging from 105 to 108 cells/0.1ml by 4 days of incubation. The survival of H. equigenitalis in liquid or semisolid media depend on medium and temperature. H. equigenitalis survived in all the media at -70 and -20°C for 3 years. When stored at 4 and 37°C, the bacteria survived in eugon, heart infusion, cooked meat, Brucella, and thiol broth for at least 8 weeks.
