Flow Injection Analysis Based Enzymatic Methods and Biosensors

Review

抄録

This review introduces a number of flow injection analysis based enzymic methods and biosensors for the determination of various analytes with interest in food, environmental and cosmetics analysis. Particular attention has been paid on the selectivity of the reviewed works as well as on their practicality in terms of flow injection manifold assembly, the fabrication of the enzyme electrodes and the performance of the measurements. A flexible and easy way to achieve upgrading of a typical flow injection manifold to a fully automated one, by means of resident software, is presented. Enzymatic methods based on packed-bed reactors bearing the immobilized enzyme(s) are discussed. The reviewed biosensors are based on a multi-membrane architecture consisted: i) of a cellulose acetate membrane in order to keep away possible interferants from the electrode surface, ii) the enzymic membrane and iii) an outer one, which protects immobilized enzyme(s) from leaching and microbial attack. Examples of various enzyme electrodes, published in the literature during the last decade, along with some flow amperometric cells are also presented.