The Journal of General and Applied Microbiology
Online ISSN : 1349-8037
Print ISSN : 0022-1260
ISSN-L : 0022-1260
Characterization of Leuconostoc species isolated from vacuum-packaged ham
Yimin CaiYoshimi BennoAkiko TakedaTsutomi YoshidaTamiko ItayaTakashi Nakase
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1998 Volume 44 Issue 2 Pages 153-159

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Abstract

Thirty-six isolates of Leuconostoc spp. were isolated from yellow spots that occurred on the surface of vacuum-packaged ham. All isolates were Gram-positive, catalase-negative cocci that produced gas from glucose and formed more than 90% of their lactate as D(−) isomer. These isolates could grow at 4°C but not above 30°C and most strains produced yellow spots on the ham. The isolates were divided into three groups by sugar fermentation patterns. Representative strains from three groups showed intergroup DNA homology values of above 88.8%, showing that these groups were composed of a single species. This organism was positioned at a separate branch in the genus Leuconostoc on the phylogenetic tree based on 16S rRNA sequences, which was assigned to Leuconostoc gelidum on the basis of DNA-DNA relatedness.

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© 1998 by The Applied Microbiology, Molecular and Cellular Biosciences Research Foundation
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