Application of the partial 16S rDNA sequence as an index for rapid identification of species in the genus Bacillus

Keiichi Goto; Tomoko Omura; Yukihiko Hara; Yoshito Sadaie

doi:10.2323/jgam.46.1

Application of the partial 16S rDNA sequence as an index for rapid identification of species in the genus Bacillus

Keiichi Goto, Tomoko Omura, Yukihiko Hara, Yoshito Sadaie

著者情報

キーワード: Bacillaceae, genus Bacillus, grouping, identification, 16S rRNA gene

ジャーナルフリー

2000 年 46 巻 1 号 p. 1-8

DOI https://doi.org/10.2323/jgam.46.1

詳細

抄録

A comparison of 16S rRNA gene (rDNA) sequences was made among type strains of 69 Bacillus species approved in the International Journal of Systematic Bacteriology (IJSB) until 1998. The results suggested that 5′ end region (approx. 275 bp) was the hypervariant region (HV region) in the gene and was highly specific for each type strain. Furthermore, a sequence analysis of the HV region of Bacillus strains revealed that this region was highly conserved within the species. These results indicate that the HV region is a useful index for the identification or grouping of Bacillus species.

責任著者(Corresponding author)

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