2000 Volume 46 Issue 2 Pages 59-67
Growth characteristics of the aerobic bacterial strain Arthrobacter citreus BI-100 in mineral salts medium with γ-hexachlorocyclohexane (γ-HCH) as the sole source of carbon and degradation of γ-HCH by the strain are reported. The highest yield of the bacteria is observed at a γ-HCH concentration of 100 mg/L. At this concentration, the bacteria entered the exponential phase of growth without any lag. At 8 h of growth, no residual HCH, but its metabolites, was detectable in the medium. The bacterium attained its stationary phase at 48 h and at 72 h; no metabolite of γ-HCH could be detected by gas chromatography. Six metabolic intermediates of γ-HCH produced by A. citreus BI-100 at different periods of growth were characterized by using gas chromatography-mass spectrometry and high-performance liquid chromatography, which furnished evidence for the presence of γ-1,3,4,5,6-pentachlorocyclohexene, tetrachlorocyclohexene, trichlorocyclohexa-diene, 2-chlorophenol, phenol, and catechol, among others.