The Journal of General and Applied Microbiology
Online ISSN : 1349-8037
Print ISSN : 0022-1260
ISSN-L : 0022-1260
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Isolation and characterization of mesophilic cellulose-degrading bacteria from flower stalks-vegetable waste co-composting system
Wen-Jing LuHong-Tao WangShi-Jian YangZhi-Chao WangYong-Feng Nie
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2005 Volume 51 Issue 6 Pages 353-360

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Abstract

Fifteen mesophilic bacteria with high Cx cellulase activities were isolated and purified from a mixed-culture enriched from a flower stalks-vegetable waste co-composting system. A CMCase test showed that the enzyme activity of these isolates ranged from 7.9 to 28.0 U ml−1. Although filter paper degrading capability was low in single culture, significant synergetic cellulose degradation were detected in four groups of mixed cultures, their degradation rates were 23.5%, 26.3%, 19.4% and 24.5%, respectively. Study of morphological and physiological characters of five predominant isolates which possess high CMCase and had positive effect on synergetic cellulose degradation in mixed culture system showed that two of them were closely related to Bacillus pasteurii and Bacillus cereus, whereas the rest belong to the genus Halobacillus, Aeromicrobium and Brevibacterium, respectively.

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© 2005 by The Applied Microbiology, Molecular and Cellular Biosciences Research Foundation
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