Hydrogenophaga temperata sp. nov., a betaproteobacterium isolated from compost in Korea

Abstract

A Gram-negative, rod-shaped, non-spore-forming and motile bacterial strain TR7-01^T was isolated from a compost soil in South Korea and subjected to a polyphasic taxonomic study. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain TR7-01^T belonged to the genus Hydrogenophaga within the class Betaproteobacteria. Strain TR7-01^T exhibited 16S rRNA gene sequence similarity values of 95.0−98.3% to members of the genus Hydrogenophaga: Hydrogenophaga bisanensis DSM12412^T (98.3%), Hydrogenophaga flava DSM 619^T (97.1%), Hydrogenophaga pseudoflava ATCC 33668^T (96.8%), Hydrogenophaga intermedia S1^T (96.4%), Hydrogenophaga atypica BSB 41.8^T (95.8%), Hydrogenophaga defluvii BSB 9.5^T (95.7%), Hydrogenophaga palleronii CCUG 20334^T (95.6%), Hydrogenophaga caeni EMB71^T (95.4%) and Hydrogenophaga taeniospiralis ATCC 49743^T (95.0%). Chemotaxonomic data revealed that strain TR7-01^T possesses ubiquinone Q-8, the G+C content was 69.9 mol%, and the predominant fatty acids were 16 : 1 ω7c/15 : 0 iso 2OH, 18 : 1 ω7c/ω9t/ω12t and C_16:0, all of which corroborated our assignment of the strain to the genus Hydrogenophaga. The results of DNA-DNA hybridization and physiological and biochemical tests clearly demonstrated that strain TR7-01^T represents a distinct species. Based on these data, TR7-01^T (= KCTC 12203^T = DSM 18117^T) should be classified as a novel Hydrogenophaga species, for which the name Hydrogenophaga temperata sp. nov. has been proposed.