Enterobacter siamensis sp. nov., a transglutaminase-producing bacterium isolated from seafood processing wastewater in Thailand

抄録

A novel strain of Enterobacter, C2361^T, a Gram-negative, non-spore-forming, rod-shaped and facultative anaerobic bacterium with the capability to produce transglutaminase, was isolated from seafood processing wastewater collected from a treatment pond of a seafood factory in Songkhla Province, Thailand. Phylogenetic analyses and phenotypic characteristics, including chemotaxonomic characteristics, showed that the strain was a member of the genus Enterobacter. The 16S rRNA gene sequence similarities between strain C2361^T and Enterobacter cloacae subsp. cloacae ATCC 13047^T and Enterobacter cloacae subsp. dissolvens LMG 2683^T were 97.5 and 97.5%, respectively. Strain C2361^T showed a low DNA-DNA relatedness with the above-mentioned species. The major fatty acids were C_16:0, C_17:0cyclo and C_14:0. The DNA G+C content was 53.0 mol%. On the basis of the polyphasic evidence gathered in this study, it should be classified as a novel species of the genus Enterobacter for which the name Enterobacter siamensis sp. nov. is proposed. The type strain is C2361^T (= KCTC 23282^T = NBRC 107138^T).