Paenibacillus humi sp. nov., isolated from peat-soil

Abstract

A Gram-positive, facultative-anaerobic, rod-shaped and endospore-forming bacterium designated as strain J30-4^T was isolated from peat-soil collected in Russia. 16S rRNA gene sequence analysis revealed that strain belongs to the genus Paenibacillus, closely related to Paenibacillus terrigena A35^T (98.1%) and Paenibacillus harenae B519^T (95.5%). Chemotaxonomic analysis revealed that strain J30-4^T possessed menaquinone MK-7 as predominant quinone and diphosphatidylglycerol, phosphatidylglycerol, phosphatidyl-ethanolamine, phosphatidyl-N-methylethanolamine and several unknown lipids were detected in the polar lipid profile. The cell wall peptidoglycan was of the A1γ type (meso-diaminopimelic acid). The major fatty acids were anteiso-C_15:0 and iso-C_16:0. The DNA G+C content of J30-4^T was 55.5 mol%. Based on phenotypic, chemotaxonomic and phylogenetic data presented in this study, strain J30-4^T (= KEMC 7302-014^T = JCM 18166^T) is indicative of a new Paenibacillus species, for which the name Paenibacillus humi sp. nov., is proposed.