Roseomonas aceris sp. nov. isolated from a mono maple tree in the Shirakami Mountains in Japan

Abstract

A novel bacterial strain belonging to the genus Roseomonas was isolated from the trunk surface of a mono maple (Acer mono) tree growing in the Shirakami Mountains. The strain, designated R-1^T, was Gram-negative, non-motile, and oval-rod, and formed reddish colonies on agar plates, as has previously been described for Roseomonas species. Although motility was not observed, cells were peritrichously flagellated. Strain R-1^T preferred organic acids over carbohydrates as growth substrates. The major cellular fatty acid was C_18:1 ω7c (48.79%). Ubiquinone-10 was the major respiratory quinone. Strain R-1^T demonstrated the highest 16S rRNA gene sequence similarity with Roseomonas pecuniae N75^T (96.9%). Phylogenetic analysis based on 16S rRNA gene sequences confirmed that strain R-1^T was a member of the genus Roseomonas and formed a cluster with R. pecuniae N75^T. DNA-DNA hybridization between strain R-1^T and R. pecuniae N75^T yielded 21.7% relatedness. On the basis of its phenotypic, phylogenetic, and chemotaxonomic char­acteristics, strain R-1^T represents a novel species within the genus Roseomonas, for which the name Roseomonas aceris sp. nov. has been proposed. The type strain is R-1^T (NBRC 109410^T = DSM 26554 ^T).