The Journal of General and Applied Microbiology
Online ISSN : 1349-8037
Print ISSN : 0022-1260
ISSN-L : 0022-1260
Full Papers
A tightly inducible riboswitch system in Synechocystis sp. PCC 6803
Ryudo OhbayashiHideto AkaiHirofumi YoshikawaWolfgang R. HessSatoru Watanabe
Author information
JOURNAL FREE ACCESS
Supplementary material

2016 Volume 62 Issue 3 Pages 154-159

Details
Abstract

Cyanobacteria are photosynthetic microorganisms that serve as experimental model organisms for the study of photosynthesis, environmental stress responses, and the production of biofuels. Genetic tools for bioengineering have been developed as a result of such studies. However, there is still room for improvement for the tight control of experimental protein expression in these microorganisms. Here, we describe an expression system controlled by a theophylline-responsive riboswitch that we have constructed in the cyanobacterium Synechocystis sp. PCC 6803. We demonstrate that, in response to different theophylline concentrations, this riboswitch can tightly control green fluorescence protein expression in Synechocystis. Thus, this system is useful as a tool for genetic engineering and the synthetic biology of cyanobacteria.

Content from these authors
© 2016, Applied Microbiology, Molecular and Cellular Biosciences Research Foundation
Previous article Next article
feedback
Top