The Journal of General and Applied Microbiology
Online ISSN : 1349-8037
Print ISSN : 0022-1260
Full Papers
Directed evolution and expression tuning of geraniol synthase for efficient geraniol production in Escherichia coli
Miki TashiroAkira FujiiShigeko Kawai-NomaKyoichi SaitoDaisuke Umeno
Author information

2017 Volume 63 Issue 5 Pages 287-295


To achieve an efficient production of geraniol and its derivatives in Escherichia coli, we aimed to improve the activity of geraniol synthase (GES) through a single round of mutagenesis and screening for higher substrate consumption. We isolated GES variants that outperform their parent in geraniol production. The analysis of GES variants indicated that the expression level of GES was the bottleneck for geraniol synthesis. Over-expression of the mutant GESM53 with a 5ʹ-untranslated sequence designed for high translational efficiency, along with the additional expression of mevalonate pathway enzymes, isopentenyl pyrophosphate isomerase, and geranyl pyrophosphate synthase, yielded 300 mg/L/12 h geraniol and its derivatives (>1000 mg/L/42 h in total) in a shaking flask.

Information related to the author
© 2017, Applied Microbiology, Molecular and Cellular Biosciences Research Foundation
Previous article Next article