2025 Volume 71 Issue 2 Article ID: 2025.10.001
Methionine gamma-lyase enzyme was isolated and purified from Mucor irregularis PQ344458 fungal isolates, that obtained from plant root, the isolates were identified through observation of their colony morphological features, implementation of PCR and DNA sequencing via sanger-chain termination approach, then data of DNA sequence alignment, phylogenetic tree, percent identity was generated. Through implementation of several stages that involved using of ion-exchange chromatography, gel-filtration chromatography, ammonium sulphate, enzyme isolation and purification stages were accomplished. The enzyme extract then, was analyzed for its protein content, specific activity and Impact of pH, temperature, inhibitors and activators on its kinetics. Additionally, MTT and DPPH radical scavenging assays were carried-out to reveal information about anti-cancer and anti-oxidant activities of methionine gamma-lyase enzyme. MTT assay results of %viable cells were 15% for HeLa cells and 6.6% for U937 cells at maximum concentration of the enzyme extract. Moreover, DPPH scavenging activity results were 82% at maximum concentration.
