The Journal of General and Applied Microbiology
Online ISSN : 1349-8037
Print ISSN : 0022-1260
ISSN-L : 0022-1260
Detection of hexahydro-1,3-5-trinitro-1,3,5-triazine (RDX) with a microbial sensor
Jed O. EberlyMichael L. MayoMatthew R. CarrFiona H. CrockerKarl J. Indest
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JOURNALS FREE ACCESS Advance online publication

Article ID: 2018.08.001

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Abstract

Explosives such as hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) are common contaminants found in soil and groundwater at military facilities worldwide, but large-scale monitoring of these contaminants at low concentrations is difficult. Biosensors that incorporate aptamers μ with high affinity and specificity for a target are a novel way of detecting these compounds. This work describes novel riboswitch-based biosensors for detecting RDX. The performance of the RDX riboswitch was characterized in Escherichia coli using a range of RDX concentrations from 0–44 μmol l–1. Fluorescence was induced at RDX concentrations as low as 0.44 μmol l–1. The presence of 4.4 μmol l–1 RDX induced an 8-fold increase in fluorescence and higher concentrations did not induce a statistically significant increase in response.

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© 2019, Applied Microbiology, Molecular and Cellular Biosciences Research Foundation
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