Elucidation of prazosin biodegradation by isolated Bacillus spp. from the tropical environment

Nur'Aqilah Farhanah Mohd Mohsi; Atiqqoh Apandi; Megat Johari Megat Mohd Noor; Fazrena Nadia MD Akhir; Norio Sugiura; Motoo Utsumi; Nor'azizi Othman; Zuriati Zakaria; Hirofumi Hara

doi:10.2323/jgam.2019.04.001

This article has now been updated. Please use the final version.

Elucidation of prazosin biodegradation by isolated Bacillus spp. from the tropical environment

Nur'Aqilah Farhanah Mohd Mohsi, Atiqqoh Apandi, Megat Johari Megat Mohd Noor, Fazrena Nadia MD Akhir, Norio Sugiura, Motoo Utsumi, Nor'azizi Othman, Zuriati Zakaria, Hirofumi Hara

Author information

Nur'Aqilah Farhanah Mohd Mohsi
Department of Environmental Engineering and Green Technology, Malaysia-Japan International Institute of Technology, Universiti Teknologi Malaysia
Atiqqoh Apandi
Department of Environmental Engineering and Green Technology, Malaysia-Japan International Institute of Technology, Universiti Teknologi Malaysia
Megat Johari Megat Mohd Noor
Department of Environmental Engineering and Green Technology, Malaysia-Japan International Institute of Technology, Universiti Teknologi Malaysia
Fazrena Nadia MD Akhir
Department of Environmental Engineering and Green Technology, Malaysia-Japan International Institute of Technology, Universiti Teknologi Malaysia
Norio Sugiura
Department of Environmental Engineering and Green Technology, Malaysia-Japan International Institute of Technology, Universiti Teknologi Malaysia
Graduate School of Life and Environmental Science, University of Tsukuba
Motoo Utsumi
Graduate School of Life and Environmental Science, University of Tsukuba
Nor'azizi Othman
Department of Mechanical Precision Engineering, Malaysia-Japan International Institute of Technology, Universiti Teknologi Malaysia
Zuriati Zakaria
Department of Environmental Engineering and Green Technology, Malaysia-Japan International Institute of Technology, Universiti Teknologi Malaysia
Hirofumi Hara
Department of Chemical Process Engineering, Malaysia-Japan International Institute of Technology, Universiti Teknologi Malaysia

Keywords: biodegradation, hospital wastewater, pharmaceutical residues, prazosin

JOURNAL FREE ACCESS Advance online publication
Supplementary material

Article ID: 2019.04.001

DOI https://doi.org/10.2323/jgam.2019.04.001

The final version of this article is now available: Vol. 66 (2020), No. 1 pp. 8-14

Details

Abstract

Prazosin (PRZ), a drug used to treat hypertensive patients, is an emergent contaminant in water systems. PRZ is an alpha-adrenergic receptor blocker used to treat anxiety, and is believed to reach the environment through human excretion, irresponsible disposal of unused medicine, and waste products from manufacturing plants. The purpose of this research was to isolate and characterize potential microbes for PRZ biodegradation and to identify the degradation pathway. After screening, isolated strain STP3 showed a capability for PRZ degradation and was chosen for further analysis. Resting cell assays with PRZ were conducted to identify the intermediate metabolites formed from biodegradation by Fourier transform ion cyclotron resonance mass spectrometer (FT-ICR MS) analysis. Two metabolites degraded from PRZ by STP3 were successfully found, and as these metabolites are derived from the main structure of PRZ, their presence proved PRZ degradation. Draft genome sequencing analysis of STP3 was performed to identify potential enzymes for PRZ biodegradation based on the metabolites found.

Corresponding author

Register with J-STAGE for free!