Abstract
The growth of a strain of the glutamic acid producing bacterium Micrococcus glutamicus remained at very low levels even when the incubation was continued for several days. The addition of both casamino acids and ferrichrome to the synthetic medium was necessary to increase the growth level of the bacterium.
Ferrichrome could be replaced by the following compounds: ferrichrome A, hydroxyaspergillic acid, large amounts of ascorbic acid or araboascorbic acid, and large quantities of iron salts.
Cysteine or cystine, histidine, leucine and any one of the aromatic amino acids were concluded to be most important amino acids. The mixture of these four amino acids could replace casamino acids.
