IS DEOXYRIBONUCLEIC ACID OF ASPERGILLUS ORYZAE CONIDIA MODIFIED CHEMICALLY IN THE EARLY PERIOD OF GERMINATION?

Abstract

The conidia of Aspergillus oryzae germinated in the presence of ¹⁴CO₂ were collected after 45min, when appreciably no net synthesis of nucleic acids proceeded, and nucleic acids were extracted with phenol from the conidia. The separation of nucleic acids were effected by various methods, such as methylated albumin-kieselguhr column chromarography, zone electrophoresis and gel-filtration chromatography.
It was clearly demonstrated that sRNA and rRNA were highly labeled while DNA was not. This results together with the previous observation on DNA base composition suggested that appreciably no modification of DNA occurred in the early period of germination.