EFFECT OF SUCCINATE ON CELLULOSE DECOMPOSITION BY THE RUMINAL BACTERIA

I. STIMULATIVE EFFECT OF SUCCINATE ON THE CELLULASE FORMATION IN CELLULOMONAS FIMI ISOLATED FROM SHEEP RUMEN

Abstract

1) Succinate was not the activator for cellulase of Cellulomonas fimi strain R2, but the cellulase biosynthesis in the cells of this strain was stimulated by succinate.
2) In the aerobic culture in a synthetic medium, the effect of succinate on the growth and the cellulase formation were inhibited by fluoroacetate.
3) In the nutritionally rich medium, the stimulative effect of succinate was observed in both aerobically and anaerobically grown cells.
4) The results suggested that succinate affected the cellulase formation not only as the energy source through the tricarboxylic acid cycle, but also by another unknown way