The Journal of General and Applied Microbiology
Online ISSN : 1349-8037
Print ISSN : 0022-1260
ISSN-L : 0022-1260
SPECTROPHOTOMETRIC STUDIES ON THE SPORULATION OF BACILLUS MEGATERIUM
TOSHIO YAMAKAWAKO AIDATEIJIRO UEMURA
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1966 Volume 12 Issue 4 Pages 353-359

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Abstract

Spectrophotometric studies in visible, ultraviolet and infrared regions of sporulating Bacillus megaterium were performed using intact cells, cell free extracts, hot-ethanol soluble extracts and dried cells of various growth phases.
In the sporulating stage, the following characteristic changes of intracellular components were suggested: (1) the marked increase in the terminal electron transport system, (2) probable change in chelating metal ion with dipicolinic acid from the stage of sporulating cell to that of matured spore, and (3) decrease of poly-β-hydroxybutyrate prior to sporulation.

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