Abstract
An attempt was made to improve the spore-forming ability of Sporolactobacillus inulinus whose sporulation rate was usually only 10-4-10-6 in a population.
An improved medium in which sporulation takes place reproducibly at the rate of 1% has been prepared by examining the effect of various substances on sporulation. From cells grown in the sporulation medium, clean spores were separated and the following features characteristic to spores of Bacillaceae were demonstrated: (1) spores could tolerate against the heat treatment at 80° for 10min, (2) electron micrographic profile of the section of the spores was characteristic to that of bacterial spores, and (3) they contained dipicolinic acid at the concentration of 5.16% dry weight.
