The Journal of General and Applied Microbiology
Online ISSN : 1349-8037
Print ISSN : 0022-1260
ISSN-L : 0022-1260
STUDIES ON ATP DEAMINASE
IV. DISTRIBUTION OF ATP DEAMINASE, AND THE PURIFICATION AND PROPERTIES OF ACID-TYPE ADP-DEAMINATING ENZYME
SHIAU-TA CHUNGKÔ AIDATEIJIRO UEMURA
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1967 Volume 13 Issue 4 Pages 335-347

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Abstract

The distribution of deaminases active for adenosine compounds was studied and it was found that besides two kinds of deaminases, i.e., non-specific ATP and adenosine deaminases, another acid-type ADP-deaminating enzyme was present in Aspergillus species.
Deaminases from Aspergillus glaucus and A. repens were purified and it was confirmed that they were of the same type of non-specific ATP deaminase which was found in Microsporum audouini by the authors.
Non-specific acid ADP-deaminating enzymes were found and purified from the koji cultures of both A. ochraceus and A. melleus. They deaminate ADP, ATP, adenosine sulfate, adenosine tetraphoshate, 5′-AMP, dAMP, 3′, 5′-cyclic AMP, adenosine, and 3′-AMP in this order. The optimum pH of ADP-deaminating enzyme obtained from both strains were 3.4 in acetate buffer, and the Km values of A. ochraceus and A. melleus were calculated to be 4.0×10-5M and 6.6×10-5M, respectively.

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