The Journal of General and Applied Microbiology
Online ISSN : 1349-8037
Print ISSN : 0022-1260
ISSN-L : 0022-1260
POLYOL DEHYDROGENASES IN CANDIDA ALBICANS
I. REDUCTION OF D-XYLOSE TO XYLITOL
L.A. VEIGA
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1968 Volume 14 Issue 1 Pages 65-78

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Abstract
Candida albicans grown in a liquid medium containing 0.5% D-xylose produced an adaptative NADP-polyol dehydrogenase which catalyzes the reversible reduction of some aldoses to the corresponding polyols. This enzyme was extracted from the culture and purified 134-fold using ammonium sulfate precipitations followed by calcium phosphate gel column chromatography. The Michaelis constant for D-xylose was determined to be 6.7×10- 3M. Heating of the enzyme up to 43° enhanced its activity, but it became completely inactive at 65°.
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