Abstract
Following the previous study on the dynamical behavior of a chemostatic culture of Azotobacter vinelandii, the microbial response to a delta type of pulse effected in a medium via glucose was studied experimentally in this account.
The glucose concentration in the medium and concentrations of cellular material and its constituents-DNA, RNA, protein, polysaccharide, and so forth-were fully analysed in unbalanced growth which immediately followed the delta pulse imposed.
The fraction of RNA in the cells was confirmed to increase sigmoidally, while the protein fraction decreased exponentially as an approximation, other cellular components remaining approximately unchanged in the unbalanced growth.
A plausible definition of unbalanced end balanced growth of microbial cells was made possible thus far. Employing the RNA fraction as parameter, a set of equations was proposed to represent the dynamical behavior of the bacterium.
