The Journal of General and Applied Microbiology
Online ISSN : 1349-8037
Print ISSN : 0022-1260
ISSN-L : 0022-1260
CYTOLOGICAL AND PHYSIOLOGICAL STUDIES ON PHOSPHATASES IN DEVELOPING CULTURES OF ASPERGILLUS NIGER
SENKICHI NAGASAKI
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1968 Volume 14 Issue 3 Pages 263-277

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Abstract

Using Aspergillus niger 1617 as a test organism, the intracellular distribution of acid and alkaline phosphatases in growing hyphae and fruiting bodies was studied cytochemically and biochemically.
Microscopical examination of the thin-layer cultures, devised specially for the present purpose, enabled distinction of younger from older hyphae. In younger hyphae, alkaline phosphatase was localized mainly in the nuclei, whereas in older ones it became dispersed in cytoplasm. Acid phosphatase in younger hyphae was distributed to surface structure, mitochondria, microsome, and cell sap, and that in older hyphae showed a similar distribution pattern. Both enzymes were highly active in rudimentary fruiting bodies.
DEAE-cellulose column chromatography and acrylamide-gel electrophoresis revealed that both phosphatases were composed of at least two components and the relative balance of these components changed characteristically during the course of cultural development.

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