The Journal of General and Applied Microbiology
Online ISSN : 1349-8037
Print ISSN : 0022-1260
ISSN-L : 0022-1260
SIGNIFICANCE OF DNA BASE COMPOSITION IN THE CLASSIFICATION OF YEAST GENERA HANSENIASPORA AND KLOECKERA
TAKASHI NAKASEKAZUO KOMAGATA
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1970 年 16 巻 3 号 p. 241-250

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The base composition of DNA (GC content) of 31 cultures of Hanseniaspora and its imperfect counterpart Kloeckera, which represented 14 species, was studied. The GC content of these apiculate yeasts ranged from 26.8 to 38.0%. Thirty-one cultures employed were divided into 4 groups on the basis of their GC contents, and this grouping agreed with that based on biochemical criteria commonly employed in yeast taxonomy. The first group (H. osmophila-K. africana) exhibited the GC content of 37.3-38.0%, and assimilated maltose but not glycerol or calcium-2-ketogluconate. The second group (K. javanica) exhibited the GC content of 33.7-34.6% and assimilated glycerol but not maltose or calcium-2-ketogluconate. The third group (H. guilliermondii, H. uvarum-K. apiculate) exhibited the GC content of 31.0-31.7% and assimilated calcium-2-ketogluconate but not maltose or glycerol. The last group (H. valbyensis-K. japonica) exhibited the GC content of 26.8-27.6%, and did not assimilate maltose, glycerol, and calcium-2-ketogluconate. Relationship between the species of Hanseniaspora and its counterpart species of Kloeckera was discussed.
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© The Microbiology Research Foundation
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