GLYCERYL ETHER PHOSPHOLIPIDS IN SELENOMONAS RUMINANTIUM

抄録

Glyceryl ether containing phospholipid was found to exist in a strictly anaerobic bacterium, Selenomonas ruminantium. Glyceryl ether fraction was obtained from total phospholipid by LiA1H₄ and methanol-HCl treatments followed by column chromatography with silicic acid. Glyceryl ether was identified by thin-layer chromatography and infrared absorption spectrum. The molar percentage composition of glyceryl ether in total phospholipids was approximately 3. When labeled valerate or caproate was supplemented to a glucose medium, the radioactivity was incorporated into fatty alcohols of glyceryl ether side chains. The major radioactive components of glyceryl ether from ¹⁴C-valerate grown cells were C_11:1, C_9:0, and C₁₃ fatty alcohols. When cells were grown with ¹⁴C-caproate, the major radioactive glyceryl ether contained C_12:0, C_12:1, and C_10:0 fatty alcohols.