BIOSYNTHESIS OF BIOTIN BY MICROÖRGANISMS

II. BIOCHEMICAL FACTORS AFFECTING THE SYNTHESIS OF BIOTIN-VITAMERS IN PHYCOMYCES BLAKESLEEANUS

Abstract

Hexoses and maltose were suitable carbon sources in supporting the growth of Phycomyces blakesleeanus and synthesis of biotin. L-Asparagine and urea were the best nitrogen sources. Sulphur-containing amino acids inhibited the synthesis of biotin-vitamers. Some amino acids, organic acids, and Tweens stimulated the biosynthesis of biotin-vitamers. Lysine and pimelic acid were the most effective. It is assumed that some amino acids and organic acids play an important role in the biosynthesis of biotin- vitamers. Adenine increased the accumulation of true and total biotin- vitamers but other heterocyclic compounds repressed the synthesis of biotin- vitamers.