COENZYME Q SYSTEM IN THE CLASSIFICATION OF THE YEAST GENERA RHODOTORULA AND CRYPTOCOCCUS, AND THE YEAST-LIKE GENERA SPOROBOLOMYCES AND RHODOSPORIDIUM

Abstract

From the cells of 142 strains of the genera Rhodotorula, Cryptococcus, Sporobolomyces, and Rhodosporidium, coenzyme Q (Co-Q) was extracted and partially purified by thin-layer chromatography. The type of Co-Q was mainly determined by paper chromatography. The Co-Q system of these organisms, which were considered to have a phylogenetic relationship to Basidiomycetes, was characterized by the dominant distribution of Q₁₀. An exceptional distribution of Q₉ was found in several strains of Rh. glutinis whose perfect stage is Rhodosp. toruloides, Rh. glutinis var. rufusa, Rh. lactosa, Cr. neoformans var. uniguttulatus, Cr. luteolus, and Rhodosp. malvinellum. Rhodotorula infirmo-miniata was the only species with Q₈. The genus Rhodosporidium was divided into two groups composed of Q₁₀ and Q₉ possessors on the basis of their Co-Q system. These results are discussed in connection with other criteria such as serological characteristics and DNA base composition.