Abstract
1) Lipopolysaccharide with pyocin R receptor activity was isolated from Pseudomonas aeruginosa P14 by the phenol method. Lipopolysaccharide was dissociated and fractionated into amino-sugar-rich fraction and lipopolysaccharide subunits by Sephadex G-100 gel filtration after heat treatment in the presence of sodium deoxycholate.
2) The lipopolysaccharide subunits (mol. wt. 12, 000-16, 000) had no receptor activity in the presence of sodium deoxycholate, but they were reassociated in the absence of sodium deoxycholate and the activity was recovered. Therefore, the subunits may be regarded as the chemical entity of the receptor activity.
3) Chemical analysis of the reassociated lipopolysaccharide and the carbohydrate fragment (mol. wt. 1, 300-1, 500) after acid treatment for the elimination of "lipid A" was carried out. The former contained glucose, rhamnose, heptose, glucosamine, galactosamine, quinovosamine, fucosamine, and a 2-keto-3-deoxy-sugar acid. The latter contained only glucose, rhamnose, heptose, galactosamine, 2-keto-3-deoxy-sugar acid, and phosphate.
