BACTERIAL LYSIS OF CLOSTRIDIUM. SPECIES

IV. FRADIOMYCIN AND SOME OTHER ANTIBIOTICS ARE SPECIFIC INHIBITORS AGAINST ENDOLYSIN OF AN INDUCIBLE BACTERIOCIN

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Lysis of Clostridium saccharoperbutylacetonicum was induced by mitomycin C treatment, with a concomitant production of bacteriocin (clostocin O) and its endolysin. The cellular lysis produced by the endolysin was drastically stopped by the addition of fradiomycin (neomycin) at above 100μg/ml. In an in vitro reaction system, fradiomycin also inhibited the action of isolated endolysin upon the Formalin-treated organisms and cell wall used as the substrate of Cl. Saccharoperbutylacetonicum. Therefore, fradiomycin seemed to be a specific inhibitor against the activity of clostocin O-endolysin.
Similar effects were observed with kanamycin, novobiocin, streptomycin, viomycin, and polymyxcin B.