GENETIC MAPPING OF PYOCIN R3 FACTOR IN PSEUDOMONAS AERUGINOSA

Abstract

A genetic character of a pyocinogenic factor responsible for the production of pyocin R3 of Pseudomonas aeruginosa was studied. This factor could be transferred from the original strain PAT to PAO strains by FP2-mediated conjugation or by transduction with a bacteriophage. The behavior of pyocin R3 factor was compared with that of pyocin R2 factor. The results indicated both pyocinogenic factors to be linked to a certain tryptophan marker. The exchange of pyocin factors was observed when the pyocin R3 factor was introduced into pyocin R2-positive recipients. It is concluded that pyocin R3 factor is integrated into the bacterial chromosome at a corresponding position of pyocin R2, close to trp-1-str region.