抄録
Colonies of microorganisms capable of forming curdlan-type polysaccharide in glucose medium were found to stain blue with Aniline Blue, Brilliant Blue, or Trypan Blue, and red with Congo Red. Staining with Aniline Blue was highly specific for colonies forming curdlan-type polysaccharide. With the use of Aniline blue to detect curdlan-type polysaccharide and other β-1, 3-glucans, the distribution of these polysaccharides in microorganisms was investigated. Among 687 strains of bacteria, 5 strains of Bacillus stained with Aniline Blue, in addition to Alcaligenes faecalis var. myxogenes 10C3, some strains derived from it, and some strains of Agrobacterium. Sixteen of 60 strains of yeast also stained with this dye. No positive reaction was detected in 13 strains of fungi tested. This method was also useful for isolation of colonies forming only curdlan-type polysaccharide from organisms with the ability to form other water-soluble polysaccharides.
