抄録
A phage tail-like bacteriocin, clostocin O, from Clostridium saccharoperbutylacetonicum (ATCC 13564) was purified by two-phase fractionation and sucrose density gradient centrifugation. Clostocin O had two kinds of particle form; one is the intact form (sheathed form) and the other is an inactive form (contracted form). A part of the intact form converted into the contracted form during the purification. The s20, w of clostocin O was 95S for the intact form and 74S for the contracted form. The purified intact clostocin O was homogeneous by several criteria such as sedimentation pattern of sucrose density gradient, sedimentation velocity, and isoelectric focusing. The isoelectric point was pH 4.6. The intact clostocin O mainly consisted of protein, but contained some (2-3%) RNA. Its amino acid composition was also determined with an amino acid analyzer.
