Abstract
Pure culture isolation of aerobic chemoheterotrophic spirilla from mud and sand samples collected from various freshwater and seawater areas in Japan was accomplished by applying the following three methods in the sequence shown: (i) boiled shellfish infusion method for enrichment of spirilla; (ii) glass capillary method for selection of spirilla; and (iii) ordinary streak plate method for pure culture isolation of spirilla. The aerobic spirilla grew well in all enrichment cultures in the 20 experiments performed. Crude cultures containing abundant spirilla were obtained from all enrichment cultures by use of glass capillaries. Pure culture isolation of spirilla from the crude cultures was reliably achieved by the ordinary streak plate method.
